Evaluation of LAMP by detecting L2 stage D. immitis in mosquitoes. D. immitis in L2 stage were counted under microscopy prior to DNA extraction. (A-F) Mosquito Malpighian tubules infected with D. immitis in L2 stage. Figure in parentheses indicates the number of D. immitis. (N) Malpighian tubules from intact mosquito. White arrowheads indicate D. immitis in mosquito Malpighian tubules. (G and H) LAMP detection of D. immitis by electrophoresis (G) and real-time turbidimeter (H) with genomic DNA from mosquitoes corresponding to (N)-(F) shown left. Numbers on the left indicate migration of molecular weight marker (bp). (I) An example of mosquito Malpighian tubules infected with D. immitis L2 larvae at 200-fold magnification.