Absence of Ac -FTT-2 in excretory/secretory products of adult and L3 Ancylostoma caninum. ESP from 6000 non-activated or activated L3, and 10 μg of adult ESP were separated by 4–20% gradient SDS-PAGE and transferred to PVDF membrane for Western blotting. A. Lane 1, non-activated L3 ESP; lane 2, activated L3 ESP; lane 3, adult ESP; lane 4, lysate (20 μg) of HEK293 expressing Ac-FTT-2. The blot was probed with anti-14-3-3β antibody. B. Control gels. Lane 1, non-activated L3 ESP; lane 2, activated L3 ESP; lane 3, recombinant ASP-1 (80 ng); lane 4, adult ESP; recombinant TIMP-1 (130 ng). Blots were probed with anti-ASP-1 antiserum (left) or anti-TMP-1 antiserum (right).