Intraclonal mating occurs during tsetse transmission of Trypanosoma brucei

Parasites & Vectors

Table 3 Genotypes and DNA contents of clones from 1738 RGFP cross

Clone	Fluorescence	DNA content^a	Microsatellite locus^b
			II-PLC	III-2	IV-TB4/7	VI-TB6/1	VIII-TB8/9	IX-TB9/6	X-TB10/17	XI-53
1738 RFP	Red	0.91	cd	cd	cd	bc	bc	cd	bc	bc
1738 GFP	Green	0.91	cd	cd	cd	bc	bc	cd	bc	bc
Fly 1 clones 1-8	Red	0.90	cd	cd	cd	bc	bc	cd	bc	bc
Fly 6 clone 1	None	0.97	cd	cd	cd	bc	bc	cd	bc	bc
Fly 6 clone 2	Green	0.93	cd	cd	cd	bc	bc	cd	bc	bc
Fly 6 clone 3	Green	0.90	cd	cd	cd	bc	bc	cd	bc	bc
Fly 6 clone 4	Green	0.90	cd	cd	cd	bc	bc	cd	bc	bc
Fly 6 clone 5	Red	0.91	cd	cd	cd	bc	bc	cd	bc	bc
Fly 6 clone 6	Green	0.96	cd	cd	cd	bc	bc	cd	bc	bc
Fly 7 clone 1	Red	1.21	cd	cd	cc	cc	bc	dd	bb	bc
Fly 7 clone 2	Red^c	0.98	cd	cd	cc	cc	bc	dd	bb	bc
Fly 7 clone 3	None	1.17	cd	cd	cd	bc	bc	cd	bc	bc
Fly 7 clone 4	None	0.96	cd	cd	cc	bb	bc	cd	bc	bb
Fly 7 clone 5	None	0.95	cd	cd	cc	bb	bc	cd	bc	bb
Fly 7 clone 6	Red	0.97	cd	cd	cc	cc	bc	dd	bb	bc

a. DNA content relative to T. b. brucei strain 427, arbitrarily assigned a DNA content of 1.00. For Fly 1, value given is for clone 1.
b. Primers for microsatellite loci on chromosomes II, III and XI given in [8], and on chromosomes IV, VI, VIII, IX and X from database of Dr Annette Macleod http://www.gla.ac.uk/centres/wcmp/research/macleod/trypanosomegeneticmappingdatabase/ text indicates differences from alleles of T. b. brucei strain 1738.
c. Two copies of mRFP gene

ISSN: 1756-3305