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Figure 1 | Parasites & Vectors

Figure 1

From: A luciferase based viability assay for ATP detection in 384-well format for high throughput whole cell screening of Trypanosoma brucei brucei bloodstream form strain 427

Figure 1

Detection of exogenous ATP with the luciferase assay. (A) Fifty-five μL of ATP in HMI-9 medium detected by 15 μL of CellTiter-Glo reagent over a dose range resulting in a plateau of the signal at 400 μM. (B) Representation of a linear range of ATP detection with CellTiter-Glo over varying concentrations of ATP. Linearity was approximately 40 μM before the signal plateau. Each concentration was screened in triplicate. The experiment was repeated twice.

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Parasites & Vectors

ISSN: 1756-3305

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