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Table 1 Luciferase assay estimated activities over varying T.b.brucei inocula for the reference compounds staurosporine and diminazene.

From: A luciferase based viability assay for ATP detection in 384-well format for high throughput whole cell screening of Trypanosoma brucei brucei bloodstream form strain 427

Cell density/mL

Staurosporine

Diminazene

2000

13.50 ± 8.5

453.8 ± 130.2

1500

19.90 ± 1.3

138.1 ± 4.4

1000

8.73 ± 3.2

178.2 ± 57.7

500

7.60 ± 0.6

123.4 ± 6.4

250

7.96 ± 1.2

61.80 ± 7.1

  1. IC50values for each reference compound/cell inoculum concentration are expressed in ± standard deviation, in nM.
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Parasites & Vectors

ISSN: 1756-3305

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