Distribution of Brugia malayi larvae and DNA in vector and non-vector mosquitoes: implications for molecular diagnostics

Table 1 Distribution of B. malayi larvae in vector and non-vector mosquitoes as assessed by dissection.

	Time post ingestion	Number of parasites recovered^a				Total worms^a	Percentage of mosquitoes harboring parasites
		Midgut	Abdomen	Thorax	Head
Ae. aegypti-LVP	2 h	23	21	43	n.d.	87	93% (6.2 ± 6.1)^b
	7 d	0	0	65	0	65	73% (5.9 ± 5.9)
	14 d	n.d.	7	30	22	59 (20)	80% (3.9 ± 3.2)
Cx. pipiens	2 h	100	4	0	n.d.	104	93% (7.5 ± 7.1)
	7 d	0	4	0	0	4	13% (2.0 ± 1.4)
	14 d	0 (10)	0	0	0	0	0%

^aParasites were observed in mosquito dissections by microscopy at various times after ingestion of microfilaremic blood. Fifteen mosquitoes were dissected at each time point, except as noted (n.d., 10, or 20).
^bMosquito infection rate (mean intensity ± SD)