Table 2 Primers, probes and conditions used for semi-quantitative real-time PCR
From: Dynamics of digestive proteolytic system during blood feeding of the hard tick Ixodes ricinus
Product | Forward primer 5'-3' | Reverse primer 5'-3' | Annealing Temp. (°C) | Probe No.a | Amplicon size (bp) |
|---|---|---|---|---|---|
ELF1A | acgaggctctgacggaag | cacgacgcaactccttcac | 60 | 165 | 81 |
IrCL | aaccacctggggtgatga | caagaggtatgctagcactgga | 60 | 15 | 89 |
IrCD | gacagaaggcggacagtacc | cggaaattgtgaaggtgacat | 60 | 78 | 74 |
IrAE | cgaaaccgtgctttcctg | tcagtcttctcagcgtcacc | 60 | 22 | 77 |
IrCB | tcaacaagatcaacacaacttgg | tcatggagatggatttgtcg | 60 | 4 | 60 |
IrCC | caccaagaacagggtgaagaa | ctcgcaaccctgagagtagg | 60 | 15 | 76 |