Figure 3
Lectin blotting with a range of lectins to An. stephensi 43 cell line glycoproteins before and after in situ glycosidase treatments. The position of molecular weight markers run concurrently are shown, values in kDa. Two μg of protein were loaded to each well. Figure 3A: Lane 1 SNA, after neuraminidase (lane 2); lane 3 RCAII, after β-galactosidase (lane 4), after PNGase F (lane 5); lane 6 Con A, after PNGase F (lane 7), after Endo H (lane 8); lane 9 PNA, after O-glycanase™ (lane 10), after PNGase F (lane 11); lane 12 DBA, after α AGA (lane 13), after PNGase F (lane 14); lane 15 JAC, after PNGase F (lane 16). Figure 3B: Lane 1 AAL, after Endo H (lane 2), after PNGase A (lane 3); lane 4 Con A, after PNGase A (lane 5), after periodate (lane 6). Representative blot shown of 3-5 separate experiments.