Figure 3

Probing of the schistosome protein microarray. The protein microarray includes cell-free S. japonicum (Sj) and S. mansoni (Sm) proteins as well as known recombinant antigens (RA) that were expressed conventionally in vivo in bacteria and yeast. The protein microarray was probed with monoclonal antibodies against the N-terminal poly-histidine tag (A) and C-terminal hemagglutinin tag (B); with pooled sera from Swiss outbred mice after a 7 week infection with S. japonicum (C); S. mansoni (D); and naïve mouse serum (E).