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Table 3 Serological and PCR results for eight patients infected with Bartonella koehlerae.

From: PCR amplification of Bartonella koehlerae from human blood and enrichment blood cultures

Patient Date IFA Reciprocal Titers PCR Results
   B. koehlerae B. henselae Bvb II R. rickettsii ITS PCR rpoB PCR
  10-2008 64 <16 <16 <16 Bvb*,a Neg
1 02-2009** <16 <16 <16 <16 B. koehlerae a B.koehlerae a
1 04-2009** <16 <16 <16 <16 Bsppb Neg
2 03-2009 <16 <16 32 64 B. koehlerae c B.koehlerae a
2 04-2009 NA NA NA NA Neg Neg
2 07-2009** <16 <16 <16 16 B. koehlerae a Neg
2 10-2009** <16 <16 <16 <16 Neg Neg
3 03-2009 64 <16 64 <16 B. koehlerae a B.koehlerae a
3 04-2009 NA NA NA NA B. koehlerae c Neg
3 05-2009** 64 <16 32 <16 Neg Bvb*,a
3 08-2009** 256 <16 16 <16 Neg Neg
3 09-2009** <16 <16 <16 <16 Neg Neg
4 4-13-2009 <16 <16 <16 32 B. koehlerae b Neg
4 4-30-2009** <16 <16 <16 <16 Neg Bvb*,a
4 6-02-2009** <16 <16 32 <16 Neg Neg
4 6-09-2009** <16 <16 64 16 Neg Neg
4 6-30-2009** 64 <16 64 <16 Neg Neg
5 05-2009 NA NA NA NA B. koehlerae a B.koehlerae a
6 05-2009 NA NA NA NA B. koehlerae a B.koehlerae a
7 04-2009 64 <16 32 128 B. koehlerae b Neg
7 06-2009 64 <16 64 64 Neg Bvb*,a
7 07-2009 64 <16 <16 64 B. henselae a Neg
7 11-2009** 256 <16 <16 16 B. henselae a Neg
8 04-2009 64 <16 <16 16 B. koehlerae b Neg
8 05-2009 64 <16 <16 <16 B. koehlerae a B.koehlerae a
8 07-2009 64 <16 <16 32 Neg Neg
8 08-2009 <16 <16 32 <16 Neg Neg
  1. All ITS and rpoB PCR amplicons were sequenced to confirm genus and species identity.
  2. *Bvb = Bartonella vinsonii subsp. berkhoffii genotype II
  3. Bspp = amplicon generated with 16S-23 S ITS primers, but unable to obtain a DNA sequence.
  4. ** Denotes post-antibiotic testing. NA = Sample not available.
  5. a = amplicon sequenced from blood or serum
  6. b = amplicon sequenced from BAPGM enrichment blood culture
  7. c = amplicons sequenced from both blood and BAPGM enrichment blood culture