PCR amplification of Bartonella koehlerae from human blood and enrichment blood cultures

Parasites & Vectors

Table 3 Serological and PCR results for eight patients infected with Bartonella koehlerae.

Patient	Date	IFA Reciprocal Titers				PCR Results
		*B. koehlerae*	*B. henselae*	*Bvb II*	*R. rickettsii*	ITS PCR	rpoB PCR
	10-2008	64	<16	<16	<16	Bvb*^,a	Neg
1	02-2009**	<16	<16	<16	<16	B. koehlerae ^a	B.koehlerae ^a
1	04-2009**	<16	<16	<16	<16	Bspp^b	Neg
2	03-2009	<16	<16	32	64	B. koehlerae ^c	B.koehlerae ^a
2	04-2009	NA	NA	NA	NA	Neg	Neg
2	07-2009**	<16	<16	<16	16	B. koehlerae ^a	Neg
2	10-2009**	<16	<16	<16	<16	Neg	Neg
3	03-2009	64	<16	64	<16	B. koehlerae ^a	B.koehlerae ^a
3	04-2009	NA	NA	NA	NA	B. koehlerae ^c	Neg
3	05-2009**	64	<16	32	<16	Neg	Bvb*^,a
3	08-2009**	256	<16	16	<16	Neg	Neg
3	09-2009**	<16	<16	<16	<16	Neg	Neg
4	4-13-2009	<16	<16	<16	32	B. koehlerae ^b	Neg
4	4-30-2009**	<16	<16	<16	<16	Neg	Bvb*^,a
4	6-02-2009**	<16	<16	32	<16	Neg	Neg
4	6-09-2009**	<16	<16	64	16	Neg	Neg
4	6-30-2009**	64	<16	64	<16	Neg	Neg
5	05-2009	NA	NA	NA	NA	B. koehlerae ^a	B.koehlerae ^a
6	05-2009	NA	NA	NA	NA	B. koehlerae ^a	B.koehlerae ^a
7	04-2009	64	<16	32	128	B. koehlerae ^b	Neg
7	06-2009	64	<16	64	64	Neg	Bvb*,^a
7	07-2009	64	<16	<16	64	B. henselae ^a	Neg
7	11-2009**	256	<16	<16	16	B. henselae ^a	Neg
8	04-2009	64	<16	<16	16	B. koehlerae ^b	Neg
8	05-2009	64	<16	<16	<16	B. koehlerae ^a	B.koehlerae ^a
8	07-2009	64	<16	<16	32	Neg	Neg
8	08-2009	<16	<16	32	<16	Neg	Neg

All ITS and rpoB PCR amplicons were sequenced to confirm genus and species identity.
*Bvb = Bartonella vinsonii subsp. berkhoffii genotype II
Bspp = amplicon generated with 16S-23 S ITS primers, but unable to obtain a DNA sequence.
** Denotes post-antibiotic testing. NA = Sample not available.
^a = amplicon sequenced from blood or serum
^b = amplicon sequenced from BAPGM enrichment blood culture
^c = amplicons sequenced from both blood and BAPGM enrichment blood culture

ISSN: 1756-3305