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Figure 3 | Parasites & Vectors

Figure 3

From: Use of Multiple Displacement Amplification as Pre-polymerase Chain Reaction (Pre-PCR) to amplify genomic DNA of siphonapterids preserved for long periods in scientific collections

Figure 3

Amplification, with the use of the MDA technique, of the 200 bp fragment of the 16S mtDNA gene of samples from scientific collections preserved in 70% ethanol and stored at room temperature. 1) 100 bp DNA Marker; 2) Tunga penetrans (control); 3) Ctenocephalides felis felis (Control); 4 and 5) T. penetrans (2007 and 2002); 6 and 7) T. travassosi (1966 and 1916); 8, 9 and 10) T. caecata (2007, 1980 and 1989); 11 and 12) T. terasma (1996 and 2003); 13) T. bondari (1909); 14 and 15) Tunga sp. (2000); 16) C. felis felis (1988); 17) Negative control.

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Parasites & Vectors

ISSN: 1756-3305

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