Course of L.major infection in BALB/c mice vaccinated with ΔHSP70-II promastigotes. BALB/c mice (n = 5) were inoculated with 1 × 107 ΔHSP70-II promastigotes and, 4 weeks later, challenged with 1,000 metacyclic promastigotes of L. major. The control group (unvaccinated) was challenged with an identical inoculum of L. major. (A) Course of lesion progression. (B) Parasite burdens in spleen and popliteal lymph node (right foot). The data presented are representative of two experiments with similar results.