Table 3 Primers used for the PCRs for sequence analysis for Anaplasma phagocytophilum from horses in this study.

16S rRNA

First reaction:

ge3a: CACATGCAAGTCGAACGGATTATTC

ge10r: TTCCGTTAAGAAGGATCTAATCTCC

Nested reaction*:

ge9f: AACGGATTATTCTTTATAGCTTGCT

ge2: GGCAGTATTAAAAGCAGCTCCAGG

40 cycles (nested reaction: 25 cycles):

30 sec 94°C, 30 sec 55°C, 1 min 72°C

[42]

groEL

First reaction:

EphplgroEL-F: ATGGTATGCAGTTTGATCGC

EphplgroEL-R: TCTACTCTGTCTTTGCGTTC

Nested reaction*:

EphplgroEL-F: ATGGTATGCAGTTTGATCGC

EphgroEL-R: TTGAGTACAGCAACACCACCGGAA

40 cycles:

30 sec 94°C, 30 sec 55°C, 45 sec 72°C

[28]

Msp2

msp25: TTATGATTAGGCCTTTGGGCATG*

msp23:TCAGAAAGATACACGTGCGCCC*

35 cycles:

1 min 95°C, 1 min 62°C, 1.5 min 72°C

[43]

Msp4

First reaction:

MSP4AP5: ATGAATTACAGAGAATTGCTTGTAGG

MSP4AP3: TTAATTGAAAGCAAATCTTGCTCCTATG

40 cycles:

30 sec 94°C, 45 sec 54°C, 1 min 72°C

[30]

Nested reaction*:

msp4f: CTATTGGYGGNGCYAGAGT

msp4r: GTTCATCGAAAATTCCGTGGTA

[44]