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Table 1 Sequences of primers used to amplified T.cruzi genes using qPCR

From: Gene expression study using real-time PCR identifies an NTR gene as a major marker of resistance to benznidazole in Trypanosoma cruzi

Function Genes/Genebank ID Size Forward Reverse
Activation system Old Yellow enzyme (OYE)1/AB075599 146 bp 5'-ACTTTCGCTTGCCTATCTGC-3' 5'-GTATTTGCTGGTCTGCCTCTTC-3'
  Nitroreductase I (NTR)1/XM805552 195 bp 5'-GCACGTGATTGGTATGGATG-3' 5'-CTTTGTTGGGTCAAATCGCT-3'
  NADPH-cytochrome P450 reductase (P450)1/DQ857724 140 bp 5'-GCATACCGGTTGGACACTTT-3' 5'-GCCTTCAGGAATGATACGGA-3'
Detoxification system of free radicals Trypanothione reductase (TRYT)1/M38051 191 bp 5'-CTCTACAAGAAGCGGGTTGC-3' 5'-CTGAGAGTGGTGCGATCAAA -3'
  Glutathionyl spermidine synthetase (GTS)1/XM_815753.1 125 bp 5'-ACTTCCACCGGGTCTTTTCT-3' 5'-CTGCGGCATTCATCACATAC -3'
  Trypanothione synthetase (TS)1/XM_805507.1 130 bp 5'-ATCCGTTGGAGGATGAAGTG-3' 5'-TAAATGTCAGACGACGCAGC-3'
  Cytosolic tryparedoxin peroxidase (cTXP)2/AJ012101 169 bp 5'-AAGTGGCTGGTGCTCTTCTT-3' 5'-TTGCGCTCAATGCTTGTCCA-3'
  Mitochondrial tryparedoxin peroxidase (mTXP)2/AJ006226.1 137 bp 5'-TGCAACACCCTGCGACTTCTTA-3' 5'-GCCCTTGTAGTCATTCAAGCTG-3'
  Superoxide dismutase-A (SOD)1/U90722 184 bp 5'-GTTGAGACGTGCGGTGAATA-3' 5'-GCCTTGTGGTGTTTGGTGTA-3'
Extracellular flow of drugs system Multi-drug resistant gene (MDR)1/XM_806226.1 188 bp 5'-ATCGCTTCTATGACCCTTCCTC-3' 5'-GGCTTCCTCCACTTCTTCGT-3'
Folate metabolism system pteridine reductase-1 (ptr1)1/AF174398 157 bp 5'-ACAGTATCGCTGTGCGTCTG-3' 5'-AGAGAGCTGCCGAGACTGAG-3'
  Methionine adenosyltransferase (MAT)1/XM_799937 140 bp 5'-CTACGCCGTAATGGGACACT-3' 5'-TGTTGGGCCGAGATAAGAAC-3'
  Dihydrofolate reductase-thymidylate synthase (DHFR-TS)1/XM_810234 164 bp 5'-GTGGCGGTAAATGGTGGACT-3' 5'-ATGGTGGTGCGGTAAATAGC-3'
  Spermidine putrescine transporter (EPT)2/XM_805310.1 152 bp 5'-TGAATCCACCGTTGCGGGTCT-3' 5'-CGCATTCTTGACGCTCTGCCT-3'
Celullar metabolism system Histone H1 (H1)2/L27119.1 124 bp 5'-GCAGCAGCCAAGAAGGCTGT-3' 5'-CGACAAAGCACGGTTGAGCCA-3'
  Zinc finger protein (ZN)2/XM_808517 132 bp 5'-TGCAGCACGTTCCATTGTGCC-3' 5'-CGCTGCCGCCCGTAGTTTAAT -3'
  L-threonine dehydrogenase (TDH)2/XM_807811 166 bp 5'-TGAACCCCTCMACGGTKTACGGTGT-3' 5'-GTACATRTGAATGGCGTAGTCYGTGG-3'
  Glycoprotein gp82 (gp82)1/L14824 118 bp 5'-GCCAACGATAAAGGCAGTGT-3' 5'-GTGTGGAAGAAGCGGAGAAG-3'
  Cruzipain (CZP)2/X54414 135 bp 5'-CATCGGTGAGGTCCTCTGTT-3' 5'-CTGTGGTATGGCTGATAGCG-3'
  1. 1: Genes analyzed for all resistant phenotypes (TcR1x-TcR5x) and the parental sensitive strain
  2. 2: Genes analyzed only for the parental sensitive strain and the TcR5x resistant phenotype.