Table 2 Primers designed and suggested to amplify repeat sequence (SSRs) including number of repeats product size in bp, forward (Fw-) and reverse (Rv-) primer sequences, and melting temperature (Tm).
Name | Accession no. | SSR | Product size | Fw-Primer (5'-3') | Tm (°C) | Rv-Primer (5'-3') | Tm (°C) |
|---|---|---|---|---|---|---|---|
PPEST1 | EY218895.1 | (CA)15 | 170 | AGTTCCGCCACATCCATTC | 60.9 | TTAGACAGCGGGAAAGAAGAAA | 60.4 |
PPEST 2 | FG108562.1 | (GCA)13 | 141 | TGTCAATAGTGGCTCAATGCTC | 60.3 | ATTAGTCGTTTATCCTTCCCCG | 60.5 |
PPEST 3 | EX474573.1 | (TA)13 | 188 | CAATTTTATGCGGTCTATGGGA | 61.0 | AGGTATGCAAAGTAATGGGTGG | 60.1 |
PPEST 4 | FG116618.1 | (TC)13 | 197 | ACCCGACGCGAATTTACTTT | 60.8 | GGAGAGACAAGTTATGGGGTCA | 60.4 |
PPEST 5 | FG107376.1 | (TGC)13 | 190 | GAGAGACATGGTGGATGGACTT | 60.4 | TGTCAATAGTGGCTCAATGCTC | 60.3 |
PPEST 6 | FG108078.1 | (AT)12 | 291 | AAATCCACTATCCTCCTTTCCTC | 59.0 | TTTTGGGGTAAGATGGGG | 58.2 |
PPEST 7 | EX473561.1 | (CA)12 | 245 | GTACCCTTTCCCTCCCTATGTC | 60.1 | GGGTTCACCAACATCCTCC | 60.2 |
PPEST 8 | FG119248.1 | (CA)12 | 140 | CCACTGTAACTTGAGGAGGAGG | 60.2 | AGACTTGATGAGTGCGTCTCTG | 59.7 |
PPEST 9 | FG117610.1 | (CA)12 | 175 | CGCACAAGAACAAAGTGGAAA | 61.2 | TCTTCTCGCTCCCTCGTTC | 60.6 |
PPEST 10 | FG117371.1 | (TC)12 | 236 | ACTGAATCTTCTGCTTTCTCCATTC | 61.4 | TAAGGGAAGGGGCGGAAC | 62.2 |
PPEST 11 | ES347986.1 | (GA)11 | 162 | GGTGGATACTTGTGACGACTGA | 60.0 | CCACTCAAACTAAACTGGAAAGC | 59.4 |
PPEST 12 | FG113351.1 | (AT)10 | 233 | CTTTTCTGCCTTAGCTGCGTT | 61.0 | CGTGTCTCTTCCACCACTACAA | 60.2 |
PPEST 13 | EY206382.1 | (TC)10 | 222 | AGCTGGAATCAGGAGCAAAT | 58.9 | CAGTATCAAGCGAAAGCCG | 59.6 |
PPEST 14 | FK815057.1 | (GA)9 | 228 | ACGTGTTGTTTTCTGTGGAGTG | 60.1 | CTGGGTATTTTCTGCCTTGATT | 59.5 |
PPEST 15 | FK812013.1 | (TGA)9 | 157 | AAGAAAGGTTTGGCTTCGTGT | 60.2 | AATGGTGCTTCATCTCCTCTTC | 59.7 |
PPEST 16 | FK811085.1 | (TCA)9 | 239 | TTCTGTTCACACATCATTTCCC | 59.8 | TGTGGCTGTAATTTGACTGGAG | 60.2 |
PPEST 17 | FG116712.1 | (TGC)9 | 208 | CTGTTCAGCAAAACGAGACG | 59.6 | TCCCAAGTACAAAGACGGAACT | 60.0 |
PPEST 18 | EY216123.1 | (CT)9 | 276 | ACTTGCATACTCTTTCGCACAA | 59.9 | AAATTCATGGAAAACCTCCCTC | 60.5 |
PPEST 19 | EY210796.1 | (AC)9 | 211 | ACCCATCACCGTCTCTGC | 59.6 | TTTCCCTTGAACAACAACCAC | 59.9 |
PPEST 20 | EY210288.1 | (TC)9 | 293 | ACAGAAGAAACCATCCATTTGC | 60.4 | CCATATTCCCGATTGAGAGAGA | 60.4 |
PPEST 21 | EX474074.1 | (AG)9 | 244 | GGATTAGTGTGGCTCAAGATGG | 60.9 | GCAGGAAAATAGCAAAAGGGAT | 60.8 |
PPEST 22 | ES347170.1 | (GT)9 | 229 | ATGGGGTATTAAGGGAGAATGC | 60.4 | GGGACGTGTGTGAGTGAGATAG | 59.6 |
PPEST 23 | FG120710.1 | (GA)8 | 223 | CTGCATTTCTAATTTCGCGG | 60.7 | GGAGGAAGTGGACAGTGAAAAC | 60.0 |
PPEST 24 | FG118096.1 | (TC)8 | 129 | AGGCACATTTTGGTTGTCTTCT | 60.0 | ATTTAGGGAGTCAATAGCGCAG | 59.8 |
PPEST 25 | EY218678.1 | (TTTA)8 | 132 | CCATTCACTTCAAATCCATCCT | 60.2 | AACTGGGTGGTTGGTTGTTTT | 60.5 |
PPEST 26 | EY216313.1 | (AC)8 | 217 | GATTCCCCAGGCAAAATAAA | 58.9 | TAATCAATATGGTGGGTTCCG | 59.5 |
PPEST 27 | EY213284.1 | (TAAA)8 | 150 | TTGCTAAAGACAAGCGCAACT | 60.2 | CCATTCACTTCAAATCCATCCT | 60.2 |
PPEST 28 | EY209870.1 | (CAA)8 | 245 | GATCAAGGCGGTTAATTTCAAG | 60.0 | ACAATCCAGAAGGACGATGC | 60.1 |
PPEST 29 | FK814636.1 | (GGT)6 | 228 | TTTGTGGAGTTCGATGACTACG | 60.2 | GGACACATTCCTGTTCCAATTC | 60.6 |
PPEST 30 | EY218675.1 | (CT)9 | 373 | TTCGCTCTTTCTCTCTCTCTCC | 59.5 | ATTCTGTACGTTACCTGCCCTG | 60.4 |
PPEST 31 | EY215872.1 | (TA)11 | 400 | AAACGTGCATTCTCTGCCTAAT | 60.2 | CTCGATATTTATTTCCCCGCT | 59.5 |
PPEST 32 | EY210648.1 | (TTTA)6 | 123 | ATTCACTTCAAACCCCTCCTTT | 60.2 | AACTGGGTGGTTGGTTGTTTT | 60.5 |
PPEST 33 | FG115100.1 | (GAA)15 | 251 | ATACTCCCTCAGAACTAGCCCC | 60.0 | TTCGTCTTCTTCTTCTTCCTCC | 59.1 |
PPEST 34 | EY215687.1 | (AAAG)5 | 137 | CACCTACAGAGATGCTGGATTG | 59.8 | GGGCTAAAATGTGTCTTGACTTG | 60.0 |
PPEST 35 | EY214242.1 | (GT)15 | 245 | TAGTCACAACACACGAACCACA | 60.1 | TTAACCGTGAGAGTACCAGCAA | 59.8 |
PPEST 36 | EY203279.1 | (TTTA)6 | 123 | ATTCACTTCAAACCCCTCCTTT | 60.2 | AACTGGGTGGTTGGTTGTTTT | 60.5 |
PPEST 37 | EX474189.1 | (AT)7 | 208 | ACCGTGCAACCATTTTAAGTTC | 60.3 | AGTTATTCTTCTTCTTACTGCGCC | 59.5 |
PPEST 38 | FK811878.1 | (AG)5 | 256 | TCCAGATACTCAAGTTCCAGCC | 60.6 | TATAGCGTTCAGATCCACCAGA | 59.7 |
PPEST 39 | FG107375.1 | (CT)6 | 292 | CCCCAAAGAGAGTACACCAAAG | 60.0 | ATCAGCCAGTGTCGTATGAATG | 60.0 |
PPEST 40 | FG114532.1 | (AG)5 | 322 | TCCCAAGGCTATTAAGTCTGGT | 59.2 | GGCTATCGTGCAATTTTCTTCT | 59.8 |