Stage-specific expression of the TSP transcripts and proteins. A: The mRNA levels in eggs (E), cercaria (C), liver-stage schistosomulum (S), and adult worms (A) including female (F) and male (M) determined by semi-quantitative RT-PCR. SjGAPDH gene was used as an endogenous control. No template was a negative control (N). B: Expression of the TSP proteins in various stages was detected by Western blot using anti-rSj-TSP-#1~6 mouse sera, respectively. In addition, the protein extracts were prepared in the tegument proteins (TM) and the tegument-stripped proteins (STR). The positive control rSj-TSP-#1~6 were detected to product a specific band under the reaction of each immune sera.