Figure 5

GRA6-specific proliferation response of splenocytes from BALB/c and Kunming mice immunized with the GRA6 DNA vaccine and empty vector pcDNA3.1 vector. The mice (n = 15/group) were immunized on days 0, 14 and 28 with 100 μg pcDNA3.1-HisGRA6. Two groups of mice, one group of Kunming mice were immunized with pcDNA3.1-HisGRA6 and one group of BALB/c mice immunized with empty pcDNA3.1 were included as controls. The level of proliferation of splenocyte T cells in spleen was tested using the MTT method assay. *p < 0.05