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Figure 10 | Parasites & Vectors

Figure 10

From: Molecular cloning and preliminary function study of iron responsive element binding protein 1 gene from cypermethrin-resistant Culex pipiens pallens

Figure 10

Quantitative RT-PCR assay of IRE-BP 1. Quantitative RT-PCR assay of IRE-BP 1 mRNA in Cr-IRE and susceptible strains of Culex pipiens pallens. Quantitative RT-PCR was performed by using a Lightcycle-RNA amplification Kit SYBR Green and repeated using three independently purified RNA samples. The enhancement of fluorescence was found to be proportional to the initial concentration of template cDNA. IRE-BP 1 transcript copy numbers were normalized based on expression of the housekeeping b-actin from respective strains. Cr-IRE represents Cr-IRE strain and S represents susceptible strain. The data are presented as means ± SD. n = 3, p = 0.03.

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Parasites & Vectors

ISSN: 1756-3305

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