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Table 1 Primers used in identification of Theileria orientalis types

From: Emergence of new types of Theileria orientalis in Australian cattle and possible cause of theileriosis outbreaks

Primer Sequences Expected Size
SSU rRNA T. orientalis Type specific Forward Primer   
Ts-Ikeda 5'-AAGGATCCGTCTCTGCTACCGCCGC-3' 826bp (Kubota et al., 1996)
Ts-Chitose 5'-GCGGATCCTCATCGTCTCTGCAACT-3' 831bp "
Ts-Buffeli 5'GCGGATCCGCTCTGCAACCGCAGAG-3' 825bp "
Type specific Universal Reverse Primer   
Ts-R 5'-TGTGAGACTCAATGCGCCTA-3'  
MPSP ( Targeting entire Theileria orientalis group)   
MPSP Forward Primer 5'-CTTTGCCTAGGATACTTCCT-3' 776bp ( Ota et al., 2009)
MPSP Reverse Primer 5'-ACGGCAAGTGGTGAGAACT-3' "
SSU (Theileria orientalis)   
SSU rRNA Forward Primer 5'-ACCTGGTTGATCCTGCCAGT-3' 1800bp (Chae et al., 1998)
SSU rRNA Reverse Primer 5'-TAGGAAGACGTCCAAGTGGAATG-3' "
SSU rRNA Internal Primers (F&R) F-5' ATTGGAGGGCAAGTCTGGTG-3' 700bp (This study)
  R-5'- CTCTCGGCCAAGGATAAACTCG-3' "