Figure 5
Effect of suramin on the rate of rat liver PFK-catalyzed reaction (A) and Hill plot (B) of the data presented in (A). Figure 5A. Effect of suramin on the rate of rat liver PFK-catalyzed reaction. Suramin concentrations were nil (○), 1 (Δ) and 5 nM (●). Mg2+ concentration was constant (3.3 mM). The concentration of ATP was 0.1 mM. Enzyme concentration used was 10 μg/ml. Other conditions were same as described in Materials and Methods. Figure 5B. Hill plot of the data on the effect of suramin (0-50 nM) on the rate of rat liver PFK-catalyzed reaction. The concentrations of F-6-P, ATP and Mg2+ were 3.3, 0.1 and 3.3 mM. Other conditions were the same as described in Materials and Methods. Vi and V0 are rates of reaction in the presence and absence of suramin.