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Table 1 Sequences of the primers used to amplify the MSP-1 and MSP-2 genes of P. falciparum isolates from Pahang, Malaysia

From: Genetic diversity of Plasmodium falciparum isolates from Pahang, Malaysia based on MSP-1 and MSP-2 genes

Amplification/Gene Primer Primer sequence
Primary PCR
MSP-1 M1-OF 5- -CTAGAAGCTTTAGAAGATGCAGTATTG-3-
  M1-OR 5- -CTTAAATAGTATTCTAATTCAAGTGGATCA-3-
MSP-2 M2-OF 5- -ATGAAGGTAATTAAAACATTGTCTATTATA-3-
  M2-OR 5- -CTTTGTTACCATCGGTACATTCTT-3-
Secondary PCR
MSP-1 M1-KF 5- -AAATGAAGAAGAAATTACTACAAAAGGTGC-3-
  M1-KR 5- -GCTTGCATCAGCTGGAGGGCTTGCACCAGA-3-
  M1-MF 5- -AAATGAAGGAACAAGTGGAACAGCTGTTAC-3-
  M1-MR 5- -ATCTGAAGGATTTGTACGTCTTGAATTACC-3-
  M1-RF 5- -TAAAGGATGGAGCAAATACTCAAGTTGTTG-3-
  M1-RR 5- -CATCTGAAGGATTTGCAGCACCTGGAGATC-3-
MSP-2 M2-FCF 5- -AATACTAAGAGTGTAGGTGCARATGCTCCA-3-
  M2-FCR 5- -TTTTATTTGGTGCATTGCCAGAACTTGAAC-3-
  M2-ICF 5- -AGAAGTATGGCAGAAAGTAAkCCTYCTACT-3-
  M2-ICR 5- -GATTGTAATTCGGGGGATTCAGTTTGTTCG-3-