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Figure 3 | Parasites & Vectors

Figure 3

From: Molecular epidemiology of camel trypanosomiasis based on ITS1 rDNA and RoTat 1.2 VSG gene in the Sudan

Figure 3

Agarose gel electrophoresis of field T. evansi DNA samples (50 ng) amplified with species specific primers TeRoTat920Fand TeRoTat1070R for T. evansi based on RoTat 1.2 VSG gene. A: Lane M1: 6 band marker. All other lanes are RoTat 1.2 VSG gene positive (8, 9, 12 and 17 weak positives; 8 & 9 from South Darfur); except lane 3, 4 and 16 were shown RoTate 1.2 VSG negative from Khowai. Samples shown here are from geographically different areas. B: Lane M1 6 band marker. Lanes 2-15 have camel samples. 7 and 9 are weak positives, 12 and 14 are strong positives, rest are negative, ie. 2-6, 8, 10, 11, 13 and 15. Lane 16 and 17 are control negative and positive respectively. (All samples were from Darfur). Extra bands in both gels are non-specific bands.

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Parasites & Vectors

ISSN: 1756-3305

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