Scatter plot graphs of human IgG responses from exposed (E) and non-exposed (NE) individuals against An. gambiae (A) and Ae. aegypti (B) salivary gland (SG) protein extracts according to different preservation conditions by Enzyme-Linked ImmunoSorbent Assay (ELISA). Antibody responses are represented by aOD: mean OD value of wells with salivary antigen minus mean OD value of wells without salivary antigen. Each point shows the aOD value for a single individual. Horizontal bars show medians. Differences between the reference sample (salivary glands collected on ice and store fresh at -20°C) and other preservation conditions were tested using Wilcoxon signed-rank test. p- values are indicated only when significant differences were observed. Ref. S: reference sample; CBL: cell lysis buffer.