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Table 3 Fecal samples of infected rats and data from the three different detection methods

From: Molecular identification of Clonorchis sinensis and discrimination with other opisthorchid liver fluke species using multiple Ligation-depended Probe Amplification (MLPA)

 

Detection method

Fecal Samples no.

Microscopy detection

ITS PCR

MLPA

  

First Run

Second Run

CsPL

OvPL

OfPL

1

+

-

+

+

-

-

2

+

-

+

+

-

-

3

+

-

+

+

-

-

4

+

-

+

+

-

-

5

+

-

+

+

-

-

6

+

-

+

+

-

-

7

+

-

+

+

-

-

8

+

-

+

+

-

-

9

+

-

+

+

-

-

10

+

-

+

+

-

-

11

+

-

+

+

-

-

12

+

-

+

+

-

-

13

+

-

+

+

-

-

14

+

-

+

+

-

-

15

+

-

+

+

-

-

16

+

-

+

+

-

-

17

+

-

+

+

-

-

18

+

-

+

+

-

-

19

+

-

+

+

-

-

20

+

-

+

+

-

-

21

+

-

+

+

-

-

22

+

-

+

+

-

-

23

+

-

+

+

-

-

24

+

-

+

+

-

-

25

+

-

+

+

-

-

26

+

-

+

+

-

-

27

+

-

+

+

-

-

28

+

-

+

+

-

-

29

+

-

+

+

-

-

30

+

-

+

+

-

-

31

+

-

+

+

-

-

32

+

-

+

+

-

-

33

+

-

+

+

-

-

34

+

-

+

+

-

-

35

+

-

+

+

-

-

36

+

-

+

+

-

-

37

-

-

-

-

-

-

38

-

-

-

-

-

-

39

-

-

-

-

-

-

40

-

-

-

-

-

-

41

-

-

-

-

-

-

42

-

-

-

-

-

-

43

-

-

-

-

-

-

44

-

-

-

-

-

-

45

-

-

-

-

-

-

46

-

-

-

-

-

-

47

-

-

-

-

-

-

48

-

-

-

-

-

-

  1. CsPL: Clonorchis sinensis Padlock probe pairs
  2. OfPL: Opisthorchis felineus Padlock probe pairs
  3. OvPL: Opisthorchis viverrini Padlock probe pairs