Table 2 Primer and Probe sequences for quantification of the Wolbachia ftsZ and Onchocerca volvulus β-actin genes
Gene target | Name | Sequence 5'-3' |
|---|---|---|
O. volvulus Wol ftsZ | Forward | aggaatgggtggtggtactg |
O. volvulus Wol ftsZ | Reverse | ctttaaccgcagctcttgct |
O. volvulus Wol ftsZ | Hybridization probe | ccttgccgctttcgcaatcac |
O. volvulus β-actin | Forward | gtgctacgttgctttggact |
O. volvulus β-actin | Reverse | gtaatcacttggccatcagg |
Murine Interferon γ | Forward | tcaagtggcatagatgtggaagaa |
Murine Interferon γ | Reverse | tggctctgcaggattttcatg |