Figure 2

Time course of proteolytic activity from the four larval instars and pupae of Culex quinquefasciatus . L1, first instar larva; L2, second instar larva; L3, third instar larva; L4, fourth instar larva. Peptidase activity was detected after incubation of the gels at 37°C in 100 mM Tris–HCl (pH 7.5) for 0.5, 1, 2 or 4 h. The numbers in the middle indicate the apparent molecular masses of the peptidases, expressed in kDa.