Figure 4

Effect of peptidase inhibitors on the proteolytic profile from larval instars of Culex quinquefasciatus . The samples were pre-incubated for 30 min in the presence of each of the following inhibitors: 1 mM PMSF, 100 μM TPCK or 100 μM TLCK. Then, the proteolytic activity was detected after incubation of the gels for 2 h at 37°C in 100 mM Tris–HCl (pH 7.5). The control was processed under the same conditions but in the absence of inhibitors. The numbers on the right indicate the apparent molecular masses of the peptidases, expressed in kDa.