Figure 2
From: Validation of Aedes aegypti Aag-2 cells as a model for insect immune studies
Expression of Toll, IMD and Jak/STAT pathway marker genes after exposure of Aedes aegypti Aag-2 cells Sindbis virus. Cells were infected with Sindbis virus using a MOI of 10. After 24 hour of incubation at 28°C, the expression marker genes from the Toll (A,B), IMD(C) or Jak/STAT (D) pathways was analyzed by qPCR using RP49 gene as endogenous control. Each bar represents the relative expression and standard error of the analyzed genes, calculated as described in Methods section.