Figure 5
From: Validation of Aedes aegypti Aag-2 cells as a model for insect immune studies
Aedes aegypti Aag-2 cells are able to phagocytose. Aag-2 cells were incubated with 2 × 107 1.0 μm fluorescent red latex beads (Sigma Aldrich) for 1 hour at 28°C (A-C) or 4°C (D-F, control). After this period, cells were fixed with formaldehyde 3.7% for 30 minutes and 0.4% Trypan blue solution in phosphate-buffered saline (PBS) and observed under a fluorescence microscope. (A and D) Differential interference contrast (DIC) images. (B and E) Fluorescence image. (C and F) Merge. Magnification 100X.