Validation of Aedes aegypti Aag-2 cells as a model for insect immune studies

Parasites & Vectors

Table 1 Primers used in this study

Accession number	Gene	Primer sequence	Melting temperature (°C)	R²efficiency Curve	Primer efficiency
AAEL007696	aaREL 1 For	GACTCGTCGGAGCTGAAATC	81.1	0.9989	1.1516
AAEL007696	aaREL 1 Rev	CGGTTTGTTCAGGTTGTTGA	81.1	0.9989	1.1516
AAEL007624	aaREL 2 For	TCTGTCGGCAGATGAAGTGA	79.7	0.9999	1.1144
AAEL007624	aaREL 2 Rev	GCACTGGAATGGAGAATCAAA	79.7	0.9999	1.1144
AAEL000709	Cactus For	TCTTGCGTTGAAGTGAGTGG	79.2	0.993	1.034
AAEL000709	Cactus Rev	GACCCTCTGAAAGGGAAAGG	79.2	0.993	1.034
AAEL003841	Defensin For	GATTCGGCGTTGGTGATAGT	81.9	0.9992	0.9955
AAEL003841	Defensin Rev	TTATTCAATTCCGGCAGACG	81.9	0.9992	0.9955
AAEL010083	IMD For	TCGTCAAACTCGGTTTTCCT	78.9	0.9925	1.1068
AAEL010083	IMD Rev	TGGCGGAGTTGAAGGTAAAG	78.9	0.9925	1.1068
AAEL007768	Myd88 For	CGATGCGTTCATTTTGTTTG	76.8	0.9889	1.03
AAEL007768	Myd88 Rev	CACCGCTCAGAAATCAGCTT	76.8	0.9889	1.03
AAT45939	RP49 For	GCTATGACAAGCTTGCCCCCA	83.7	0.9879	1.2002
AAT45939	RP49 Rev	TCATCAGCACCTCCAGCT	83.7	0.9879	1.2002
AAEL007765	Serpin For	ACGTGATGGATTGGATGGAG	79.2	0.9995	1.0149
AAEL007765	Serpin Rev	GTGCCTGCACTTGTTTCTGA	79.2	0.9995	1.0149
AAEL009692	STAT For	CACACAAAAAGGACGAAGCA	75.7	1	1.1797
AAEL009692	STAT Rev	TCCAGTTCCCCTAAAGCTCA	75.7	1	1.1797
AAEL001794	TEP For	ATTTTTGACGGCTTTTGTGG	78.9	0.9992	1,0604
	TEP Rev	TGGATTACTTGCCCCACTTC

ISSN: 1756-3305