Table 1 Differentiation between Leishmania subgenera by SYBR Green melting temperatures (Tm) from amplified minicircles kDNA conserved region
Source | Samples | Origin | Melting Temperature (°C ± SD) |
|---|---|---|---|
Promastigote reference strains | L (V.) braziliensis (MHOM/BR/1975/M2903) | IOC/FIOCRUZ | 77.34 ± 0.01 |
L (V.) shawi (MCEB/BR/1984/M8408) | IOC/FIOCRUZ | 77.34 ± 0.01 | |
L (V.) naiffi (MDAS/BR/1979/M5533) | IOC/FIOCRUZ | 77.34 ± 0.01 | |
L (V.) lainsoni (MHOM/BR/1981/M6426) | IOC/FIOCRUZ | 77.37 ± 0.01 | |
L (V.) guyanensis (MHOM/BR/1975/M4147) | IOC/FIOCRUZ | 77.39 ± 0.01 | |
L (L.) infantum (Syn. L. chagasi) (MHOM/BR/1974/PP75) | IOC/FIOCRUZ | 78.95 ± 0.01 | |
L (L.) amazonensis (MHOM/BR/1977/LTB0016) | IOC/FIOCRUZ | 78.94 ± 0.00 | |
Human clinical specimens | Cutaneous leishmaniasis (skin biopsies) | Rio de Janeiro/RJ | 77.68 ± 0.38 |
Visceral leishmaniasis (peripheral blood and bone marrow aspirates) | Campo Grande/MS | 78.94 ± 0.37 | |
Field sand flies | Lutzomyia intermedia | Rio de Janeiro/RJ | 77.25 ± 0.15 |
Lutzomyia migonei | Rio de Janeiro/RJ | 77.25 ± 0.15 | |
Lutzomyia cruzi | Corumbá/MS | 78.98 ± 0.10 | |
Lutzomyia forattini | Corumbá/MS | 78.98 ± 0.10 |