Figure 1
![Figure 1](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1756-3305-5-162/MediaObjects/13071_2012_Article_653_Fig1_HTML.jpg)
Multiple sequence alignment of two putative serpins identified in the subtracted larval library with a known serpin from  R. microplus  (BmSI-7), two putative serpins from  Amblyomma maculatum  (AEO34783 and AEO32449) and two putative serpins from  Ixodes scapularis  (XP002409984 and XP002399667). The five disulfide bridges formed by 5 pairs of cysteine residues are indicated by the black lines. The numbers to the left are the position of the first amino acid (first column) within each polypeptide.