Detection of 5.8S rRNA gene of T. gondii by nest-PCR analysis on agarose gel electrophoresis (1.0%). The picture show the results of the second round of nest-PCR with the pair of inner primers. Lane M is the molecular weight marker (MW) DL-2000 (TaKaRa Biotechnology Co. Ltd) which showed bands as indicated. Lane 1 is the result of positive control with an about 433-bp fragment; Lanes 2 is the result of negetive control; Lanes 3–9,11-13,15,16 are the results of negetive samples ; Lanes 10 and 14 are the results of positive samples. Data for other 21 cat blood samples not shown but results were the same for negative samples.