Molecular characterization and ligand binding specificity of the PDZ domain-containing protein GIPC3 from Schistosoma japonicum

Table 2 Validation of the predicted ligand candidates of SjGIPC3 in the Y2H system

Bait Sequences	Code	ID	C-terminus (*S. japonicum*)	C-terminus (S. mansoni)	Protein Description	Results³	Results⁴
C-terminal tails¹	L1	ACE06921	-PDERTTTL*	-HNERTTTL*	glutamate receptor NMDA	√	√
C-terminal tails¹	L2	AAX27986	-PNHLCSDV*	-SNYLCSDV*	hypothetical protein	√	√
-[SDEC]-[STIL]-[HSNQDE]-[VIL]*²	L3	CPRT0000005506	-DESNSLHV*	-EDGNSLSA*	tetraspanin (Sj25)	×	×
	L4	CPRT0000006203	-DDRLCSQV*	-DDRLCSQV*	hypothetical protein	√	√
	L5	CPRT0000007433	-ELLTSTEV*	-ELLTSTEV*	Asparagine-rich protein	√	√
	L6	CPRT0000008390	-KSNVSLSV*	-KSNVSLSV*	putative rhodopsin-like orphan GPCR	×	×

¹: The extreme four amino acids of each unique ligand carboxyl terminus listed in Table 1 were used to BLAST on NCBI to retrieve potential ligands within taxa 6182 (S. japonicum).
²: The Tailfit software was used to search against the S. japonicum proteomic database to retrieve potential ligands with the consensus-binding sequence;
³: The validation results from Y2H assays, using the SjGIPC3-PDZ domain as bait.
⁴: The validation results from Y2H assays, using full-length SjGIPC3 as bait.
√: positive in the Y2H system; ×: negative in the Y2H system.

ISSN: 1756-3305