Figure 4
Evaluation of the IgG1 and IgG2b response in sera of mice deficient of B 2 R. ELISA assay showing anti-Nucleoside hydrolase (NH36) and anti-L.(L.) chagasi promastigote lysate IgG1 and IgG2b antibodies in B2R+/+ wild type (C57) and B2R−/− mice (KOB2) intravenously infected with 3 x 107 amastigotes of Leishmania. (L.) chagasi. Results are shown as the individual absorbency values of 1/100 diluted sera obtained from two (NH36) or one (lysate) experiment with 5 mice per treatment in each experiment, at day 30 after infection. Asterisks indicate significant differences between groups as disclosed by the Mann Whitney analysis.