Figure 3
![Figure 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1756-3305-5-264/MediaObjects/13071_2012_Article_787_Fig3_HTML.jpg)
Spots up-regulated at 3DPI in CHIKV-infected salivary glands of Ae. aegypti. 130 μg of salivary gland extract from 3 DPI CHIKV-infected mosquitoes and control mosquitoes were loaded onto 3–10 NL immobilins (18 cm). The immobilins were then deposited on the top of 12% SDS-PAGE gels. Spots were revealed using SYPRO Ruby. Gel profiles were compared using Image Master Platinum software. The spots that were found up-regulated at 3DPI are indicated by circles (fold change>1.8; Anova<0.05).