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Table 1 Overview of the methodological differences in the Ixodes ricinus studies in the Siebengebirge in 1987, 1989, 2001, 2003, 2007 and 2008

From: Abundance of Ixodes ricinus and prevalence of Borrelia burgdorferi s.l. in the nature reserve Siebengebirge, Germany, in comparison to three former studies from 1978 onwards

Methods 1987/89* 2001 2003 2007 2008
Months1 Apr-Oct May, Aug-Oct May-Nov May-Nov May-Nov
Frequency monthly weekly weekly monthly monthly
Size 100m2 100m2 225m2 100m2 100m2
Air temp.2 ≥16°C ≥16°C 8°C-25°C 15°C-25°C 15°C-23°C
Humidity2 ≥80% ≥80% 57%-74% 45%-85% 52%-85%
DNA extraction n/a3 Ammonia solution n/a Ammonia solution Chelex 100 resin solution
Borrelia detection IFA+ Simple, modified PCR [45] n/a Nested PCR [44] Nested PCR [44]
Nested PCR [44]
Modified PCR [46]
IFA [15, 38]
Borrelia genotyping n/a Reverse line blotting4[44] n/a Reverse line blotting5[44, 47] Reverse line blotting5[44, 47]
  1. *The years 1987 and 1989 are listed together because the same methods were used in both study years for the study of I. ricinus abundances and Borrelia prevalences.
  2. 1 Tick collections were carried out in the respective months (Apr = April, Aug = August, Oct = October, Nov = November).
  3. 2Air temperatures and relative humidities were measured 5cm above the ground at the study sites in all years.
  4. 3n/a = not applicable.
  5. 4B. burgdorferi s.l., B. burgdorferi s.s., B. garinii, B. afzelii and B. valaisiana were identified by reverse line blotting according to Rijpkema et al. [44].
  6. 5B. burgdorferi s.l., B. burgdorferi s.s., B. garinii and B. afzelii were identified by reverse line blotting according to Rijpkema et al. [44] and DNA probes for B. garinii, B. valaisiana, B. lusitaniae, B. spielmanii and B. bissettii were designed according to Gern et al. [47].
  7. + IFA = Immunofluorescence assay.