Methods | 1987/89* | 2001 | 2003 | 2007 | 2008 |
---|---|---|---|---|---|
Months1 | Apr-Oct | May, Aug-Oct | May-Nov | May-Nov | May-Nov |
Frequency | monthly | weekly | weekly | monthly | monthly |
Size | 100m2 | 100m2 | 225m2 | 100m2 | 100m2 |
Air temp.2 | ≥16°C | ≥16°C | 8°C-25°C | 15°C-25°C | 15°C-23°C |
Humidity2 | ≥80% | ≥80% | 57%-74% | 45%-85% | 52%-85% |
DNA extraction | n/a3 | Ammonia solution | n/a | Ammonia solution | Chelex 100 resin solution |
Borrelia detection | IFA+ | Simple, modified PCR [45] | n/a | Nested PCR [44] | Nested PCR [44] |
Nested PCR [44] | |||||
Modified PCR [46] | |||||
Borrelia genotyping | n/a | Reverse line blotting4[44] | n/a |