Female Anopheles gambiae antennae: increased transcript accumulation of the mosquito-specific odorant-binding-protein OBP2

Table 2 Expression of AGAP003306 (OBP2) and AGAP0099629 (control) relative to expression of S7 in antennae of female and male A. gambiae in three biological replicates (experiments 1, 2 and 3).

Target	Experiment	Female			Male			Relative Expression
		Cт Mean	Cт SD	ΔCт Mean	Cт Mean	Cт SD	ΔCт Mean	ΔΔCт	RQ
	1	35.55	0.105		36.51	0.257
Reference S7	2	33.31	0.335		35.04	0.125
	3	33.57	0.565		32.05	0.188
	1	29.77	0.208	-5.780	33.87	0.414	-2.634	-3.145	8.849
AGAP003306	2	29.77	0.168	-3.540	33.87	0.414	-1.170	-2.370	5.171
	3	30.85	0.091	-2.721	33.99	0.309	1.941	-4.662	25.309
	1	35.42	0.298	-0.130	36.06	0.353	-0.450	0.320	0.801
AGAP009629	2	35.55	0.105	2.239	36.56	0.390	1.518	0.721	0.607
	3	36.46	0.478	2.894	34.95	0.686	2.894	0.000	1.000

Cт = (cycle threshold) number of cycles required for the fluorescent signal to reach threshold
Cт Mean = Average Cт from triplicate values on same sample in same PCR reaction
Cт SD = Standard Deviation of triplicate Cт
ΔCт Mean = Cт MeanTarget- Cт MeanReference (target=AGAP003306 or AGAP009629; reference=S7)
ΔΔCт = ΔCт Mean_Female - ΔCт Mean_Male
RQ = Relative Quantification = 2^-(ΔΔCт)

ISSN: 1756-3305