Rabensburg virus grows on mosquito cells incubated at either 28°C or 37°C. Data points represent means of three replicates at each time point +/− standard deviation. Cells were inoculated at a MOI of 0.01 PFU/ml. LOD, limit of detection; WN02-1956, West Nile virus strain WN02-1956; RABV97-103, Rabensburg virus prototype strain 97–103; RABV06-222, Rabensburg virus strain 06–222; pfu, plaque forming units; *, p<0.001. A.) Aedes albopictus (C6/36) cells incubated at 37°C. B.) Culex tarsalis cells incubated at 37°C. C.) Aedes albopictus (C6/36) cells incubated at 28°C. D.) Culex tarsalis cells incubated at 28°C.