Figure 4

Overt cytopathic effects associated with Rabensburg virus growth on Aedes albopictus cells incubated at 37°C. At three days post inoculation no obvious pathology was observed in C6/36 cells infected with Rabensburg virus strain 97–103 (A), West Nile virus strain WN02-1956 (C), or in mock inoculated cells (D). Extensive cell fusion, giant multi-nucleated cells, and syncytia formation is evident in C6/36 cells infected with Rabensburg virus strain 06–222 (B) three days post inoculation. Cell fusion, yellow arrow; *giant multi-nucleated cell; A-D scale bar, 100 μm.