Figure 5
From: Replication of West Nile virus, Rabensburg lineage in mammalian cells is restricted by temperature
Culex flavivirus (CxFV) does not grow on Vero cells incubated at 28°C. Data points represent means of three replicates at each time point +/− standard deviation. Cells were inoculated at a MOI of 0.01 PFU/ml. The absence of CxFV growth was confirmed via RT-PCR using CxFV-specific primers. LOD, limit of detection; RABV97-103, Rabensburg virus prototype strain 97–103; RABV06-222, Rabensburg virus strain 06–222; CxFV, Culex flavivirus strain HOU24518; pfu, plaque forming units.