Development of a cDNA microarray for the measurement of gene expression in the sheep scab mite Psoroptes ovis

Parasites & Vectors

Table 1 Transcripts significantly up-regulated in the fed P.ovis mite sample

GenBank accession number	Probe ID	Sequence description	Homologous species	Minimum E-value	Fold change (fed *P. ovis vs* starved *P. ovis*)	FDR corrected p-value
FR748349	Bu_002_g12	-	-	-	1.55	0.0017
FR748466	Bu_004_b02	Beta-1,3-D-glucanase	Cryptopygus antarcticus	8.82E-22	2.32	0.00002
FR748550	Bu_005_a09	-	-	-	2.47	0.002
FR748633	Bu_005_h12	Pso o 1/Der p 1 allergen	P. ovis/D. pteronyssinus	2.36E-46	2.17	0.00001
FR748686	Bu_006_e09	Macrolide glycosyltransferase	Streptosporangium roseum	1.49E-44	1.95	0.00002
FR748740	Bu_007_b11	Der p 21 allergen	D. farinae	2.13E-36	1.82	0.0002
-	Bu_007_e02	-	-	-	1.51	0.0002
FR748952	Bu_010_f10	Myosin light chain	Haemaphysalis qinghaiensis	3.26E-57	1.53	0.0029
FR749449	Bu_017_f04	Pso o 2 group 2 allergen	P. ovis	1.49E-53	2.15	0.0025

Probe ID represents the unique identifier for the microarray probe. FDR corrected p-value shows the Benjamini & Hochberg False Discovery Rate (FDR) corrected p-value for each transcript [23]. E-value is the Expect value which describes the number of BLAST hits one can expect to see by chance for a given sequence, the lower the E-value the more significant the match.

ISSN: 1756-3305