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Figure 5 | Parasites & Vectors

Figure 5

From: Real-time PCR of the mammalian hydroxymethylbilane synthase (HMBS) gene for analysis of flea (Ctenocephalides felis) feeding patterns on dogs

Figure 5

Time-course of canine blood ingestion by fleas. Fleas were put on eight dogs, and 40 fleas were removed from each dog at the indicated time points after challenge. DNA was extracted from pools of 10 fleas (4 pools per dog and time point), HMBS targets were quantified by PCR (means ± 95% CI), and an HMBS background of 315 copies per 10 fleas was subtracted. HMBS copies per 10 fleas collected after 10 min on the dogs were significantly lower than those for 30 and 60 min (74 vs. 2703 and 2225 copies; P = 0.0001, Tukey HSD test).

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Parasites & Vectors

ISSN: 1756-3305

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