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Table 3 Microsatellite validation

From: Population genetic structure of Aedes polynesiensis in the Society Islands of French Polynesia: implications for control using a Wolbachia- based autocidal strategy

  

Locus

Site

 

AP1

AP2

AP3

AP4

AP5

AP6

AealbA9

APT1

APT2

APT6

MP

N

11

11

11

11

11

11

11

11

11

11

 

Nall

3

3

1

2

1

1

7

2

4

2

 

FIS

+0.433

-0.026

-

+0.259

-

-

+0.161

-0.053

+0.320

-0.053

 

He

0.63

0.18

-

0.48

-

-

0.65

0.17

0.65

0.17

 

Ho

0.36

0.18

-

0.36

-

-

0.55

0.18

0.45

0.18

MPA

N

15

15

15

15

15

15

15

15

15

15

 

Nall

5

3

2

2

1

2

4

2

3

2

 

FIS

-0.405

-0.087

-0.273

+1

-

-

-0.018

-0.167

+0.152

-0.077

 

He

0.72

0.25

0.37

0.24

-

0.11

0.53

0.29

0.63

0.19

 

Ho

1

0.27

0.47

-

-

-

0.53

0.33

0.53

0.2

PN

N

14

14

14

14

14

14

14

14

14

14

 

Nall

5

2

3

2

1

1

4

3

3

2

 

FIS

-0.283

+0.447

-0.490

+1*

-

-

-0.139

+0.218

+0.019

-

 

He

0.62

0.26

0.54

0.51

-

-

0.38

0.36

0.58

0.07

 

Ho

0.79

0.14

0.79

-

-

-

0.43

0.28

0.57

0.07

KN

N

18

18

18

18

18

18

18

18

18

18

 

Nall

5

4

2

2

1

2

8

2

5

2

 

FIS

+0.058

-0.204

-

+1*

-

0.03

-0.018

+0.358

+0.220

-

 

He

0.65

0.56

0.06

0.49

-

0.15

0.82

0.52

0.49

0.06

 

Ho

0.61

0.67

0.06

-

-

0.06

0.94

0.33

0.39

0.05

FT

N

15

15

15

15

15

15

15

15

15

15

 

Nall

6

3

2

2

1

1

4

3

7

2

 

FIS

-0.045

-0.479

-

+0.606

-

-

-0.067

-0.340

-0.273

-

 

He

0.64

0.64

-

0.33

-

-

0.25

0.45

0.69

0.07

 

Ho

0.67

0.93

0.06

0.13

-

-

0.27

0.6

0.87

0.06

OP

N

2

2

2

2

2

2

2

2

2

2

 

Nall

3

2

1

2

1

1

3

2

2

2

 

FIS

-0.333

+1

-

+1

-

-

-0.333

-1

-

-

 

He

0.85

0.65

-

0.65

-

-

0.85

0.65

0.5

0.5

 

Ho

1

-

-

-

-

-

1

1

0.5

0.5

RC

N

12

12

12

12

12

12

12

12

12

12

 

Nall

6

3

4

4

2

1

5

2

3

1

 

FIS

-0.186

+0.224

+0.102

+0.500

+1

-

+0.725

-0.132

-0.055

-

 

He

0.64

0.64

0.37

0.49

0.16

-

0.58

0.52

0.56

-

 

Ho

0.75

0.5

0.33

0.25

-

-

0.17

0.58

0.58

-

NAO

N

21

21

21

21

21

21

21

21

21

21

 

Nall

6

5

2

2

1

1

2

5

7

4

 

FIS

-0.081

+0.077

-0.53

+0.834*

-

-

-

-0.255

+0.200

+0.152

 

He

0.7

0.62

0.14

0.56

-

-

0.05

0.57

0.65

0.33

 

Ho

0.76

0.57

0.14

0.1

-

-

0.05

0.71

0.52

0.29

RT

N

29

29

29

29

29

29

29

15

15

15

 

Nall

5

5

2

2

1

1

7

2

5

2

 

FIS

-0.204

+0.058

+0.359

+0.208

-

-

+0.002

-0.167

+0.019

-0.037

 

He

0.58

0.59

0.16

0.39

-

-

0.55

0.52

0.61

0.13

 

Ho

0.69

0.56

0.1

0.31

-

-

0.55

0.6

0.6

0.13

TOA1

N

29

29

29

29

29

29

15

15

15

15

 

Nall

6

4

2

2

1

1

8

3

5

5

 

FIS

-0.001

-0.090

-0.018

+0.125

-

-

-0.016

-0.181

-0.135

+0.576

 

He

0.66

0.57

0.07

0.36

-

-

0.59

0.4

0.71

0.31

 

Ho

0.66

0.62

0.07

0.31

-

-

0.6

0.47

0.8

0.13

TOA2

N

16

16

16

16

16

16

16

16

16

16

 

Nall

5

3

2

2

2

1

5

2

4

2

 

FIS

-0.277

+0.540

-0.429

+1

-

-

+0.080

-0.050

-0.149

-

 

He

0.69

0.54

0.44

0.31

0.06

-

0.34

0.42

0.54

0.06

 

Ho

0.88

0.25

0.63

-

0.06

-

0.31

0.44

0.63

0.06

TOA3

N

15

15

15

15

15

15

15

15

15

15

 

Nall

5

3

1

3

1

1

4

2

3

4

 

FIS

-0.102

-0.426

-

+0.691

-

-

+0.142

-0.359

-0.011

-0.094

 

He

0.73

0.52

-

0.42

-

-

0.62

0.5

0.66

0.31

 

Ho

0.8

0.73

-

0.13

-

-

0.53

0.67

0.67

0.33

FAA

N

11

11

11

11

11

11

11

11

11

11

 

Nall

5

2

1

2

1

1

6

2

3

1

 

FIS

-0.165

+0.107

-

+0.643

-

-

+0.241

0

-0.026

-

 

He

0.47

0.51

-

0.25

-

-

0.71

0.45

0.18

-

 

Ho

0.55

0.46

-

0.05

-

-

0.55

0.45

0.18

-

MOG

N

10

10

10

10

10

10

10

9

9

9

 

Nall

4

4

2

2

1

1

5

2

4

2

 

FIS

-0.000

+0.316

-0.200

+1

-

-

-0.248

-0.455

-0.347

-

 

He

0.6

0.43

0.34

0.19

-

-

0.57

0.47

0.68

0.11

 

Ho

0.6

0.3

0.4

-

-

-

0.7

0.67

0.89

0.11

MOG2

N

18

18

18

18

18

18

18

18

18

18

 

Nall

5

4

2

3

1

2

4

2

5

1

 

FIS

-0.012

+0.163

-0.259

+0.785

-

-

+0.092

+0.329

+0.101

-

 

He

0.66

0.53

0.36

0.25

-

0.06

0.43

0.24

0.62

-

 

Ho

0.67

0.44

0.44

0.06

-

0.06

0.39

0.16

0.56

-

ILP

N

7

7

7

7

7

7

7

7

7

7

 

Nall

3

2

1

3

1

1

3

2

2

1

 

FIS

+0.217

+0.478

-

+1

-

-

-0.200

+0.625

+0.368

-

 

He

0.54

0.53

-

0.61

-

-

0.49

0.36

0.44

-

 

Ho

0.43

0.29

-

-

-

-

0.57

0.14

0.29

-

TA

N

4

4

4

4

4

4

4

4

4

4

 

Nall

5

3

1

2

1

2

2

3

3

1

 

FIS

+0.143

-0.091

-

-

-

-

+0.143

-0.600

-0.286

-

 

He

0.85

0.48

-

0.24

-

0.25

0.58

0.68

0.6

-

 

Ho

0.75

0.5

-

0.13

-

0.25

0.5

1

0.75

-

NF

N

19

19

19

19

19

19

19

19

19

19

 

Nall

4

6

1

2

1

2

2

2

4

1

 

FIS

-0.093

-0.024

-

+0.780

-

-

-0.059

-0.029

-0.026

-

 

He

0.63

0.62

-

0.24

-

0.05

0.15

0.1

0.56

-

 

Ho

0.68

0.63

-

0.05

-

0.05

0.16

0.1

0.57

-

FF

N

13

13

13

12

13

13

13

13

13

13

 

Nall

5

3

1

2

1

2

4

2

5

1

 

FIS

+0.050

+0.177

-

+1

-

-

-0.043

-0.263

+0.072

-

 

He

0.65

0.55

-

0.39

-

0.08

0.22

0.37

0.66

-

 

Ho

0.62

0.46

-

-

-

0.08

0.23

0.46

0.61

-

Total

N

279

279

279

278

279

279

265

250

250

250

 

Nall

10

8

5

6

3

3

9

5

12

7

 

FIS

-0.082

0.041

-0.183

0.675

0.663

0.01

0.054

-0.102

0.02

0.102

 

He Avg

0.66

0.50

0.29

0.37

0.11

0.12

0.49

0.42

0.58

0.18

 

Ho Avg

0.70

0.47

0.32

0.17

0.06

0.10

0.48

0.48

0.58

0.18

  1. Sample size, microsatellite variability, in breeding coefficient (FIS), Observed and expected heterozygosity for A. polynesiensis samples collected in the Society Islands, French Polynesia, 2005-2008. N = sample size, Nall = number of alleles, FIS = the inbreeding coefficient, which is a measure of the reduction of heterozygosity of a subpopulation due to non-random mating, * = significant (P < 0.026) after a Bonferroni correction for heterozygote deficit using a Fisher's exact test
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