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Table 3 Microsatellite validation

From: Population genetic structure of Aedes polynesiensis in the Society Islands of French Polynesia: implications for control using a Wolbachia- based autocidal strategy

   Locus
Site   AP1 AP2 AP3 AP4 AP5 AP6 AealbA9 APT1 APT2 APT6
MP N 11 11 11 11 11 11 11 11 11 11
  Nall 3 3 1 2 1 1 7 2 4 2
  FIS +0.433 -0.026 - +0.259 - - +0.161 -0.053 +0.320 -0.053
  He 0.63 0.18 - 0.48 - - 0.65 0.17 0.65 0.17
  Ho 0.36 0.18 - 0.36 - - 0.55 0.18 0.45 0.18
MPA N 15 15 15 15 15 15 15 15 15 15
  Nall 5 3 2 2 1 2 4 2 3 2
  FIS -0.405 -0.087 -0.273 +1 - - -0.018 -0.167 +0.152 -0.077
  He 0.72 0.25 0.37 0.24 - 0.11 0.53 0.29 0.63 0.19
  Ho 1 0.27 0.47 - - - 0.53 0.33 0.53 0.2
PN N 14 14 14 14 14 14 14 14 14 14
  Nall 5 2 3 2 1 1 4 3 3 2
  FIS -0.283 +0.447 -0.490 +1* - - -0.139 +0.218 +0.019 -
  He 0.62 0.26 0.54 0.51 - - 0.38 0.36 0.58 0.07
  Ho 0.79 0.14 0.79 - - - 0.43 0.28 0.57 0.07
KN N 18 18 18 18 18 18 18 18 18 18
  Nall 5 4 2 2 1 2 8 2 5 2
  FIS +0.058 -0.204 - +1* - 0.03 -0.018 +0.358 +0.220 -
  He 0.65 0.56 0.06 0.49 - 0.15 0.82 0.52 0.49 0.06
  Ho 0.61 0.67 0.06 - - 0.06 0.94 0.33 0.39 0.05
FT N 15 15 15 15 15 15 15 15 15 15
  Nall 6 3 2 2 1 1 4 3 7 2
  FIS -0.045 -0.479 - +0.606 - - -0.067 -0.340 -0.273 -
  He 0.64 0.64 - 0.33 - - 0.25 0.45 0.69 0.07
  Ho 0.67 0.93 0.06 0.13 - - 0.27 0.6 0.87 0.06
OP N 2 2 2 2 2 2 2 2 2 2
  Nall 3 2 1 2 1 1 3 2 2 2
  FIS -0.333 +1 - +1 - - -0.333 -1 - -
  He 0.85 0.65 - 0.65 - - 0.85 0.65 0.5 0.5
  Ho 1 - - - - - 1 1 0.5 0.5
RC N 12 12 12 12 12 12 12 12 12 12
  Nall 6 3 4 4 2 1 5 2 3 1
  FIS -0.186 +0.224 +0.102 +0.500 +1 - +0.725 -0.132 -0.055 -
  He 0.64 0.64 0.37 0.49 0.16 - 0.58 0.52 0.56 -
  Ho 0.75 0.5 0.33 0.25 - - 0.17 0.58 0.58 -
NAO N 21 21 21 21 21 21 21 21 21 21
  Nall 6 5 2 2 1 1 2 5 7 4
  FIS -0.081 +0.077 -0.53 +0.834* - - - -0.255 +0.200 +0.152
  He 0.7 0.62 0.14 0.56 - - 0.05 0.57 0.65 0.33
  Ho 0.76 0.57 0.14 0.1 - - 0.05 0.71 0.52 0.29
RT N 29 29 29 29 29 29 29 15 15 15
  Nall 5 5 2 2 1 1 7 2 5 2
  FIS -0.204 +0.058 +0.359 +0.208 - - +0.002 -0.167 +0.019 -0.037
  He 0.58 0.59 0.16 0.39 - - 0.55 0.52 0.61 0.13
  Ho 0.69 0.56 0.1 0.31 - - 0.55 0.6 0.6 0.13
TOA1 N 29 29 29 29 29 29 15 15 15 15
  Nall 6 4 2 2 1 1 8 3 5 5
  FIS -0.001 -0.090 -0.018 +0.125 - - -0.016 -0.181 -0.135 +0.576
  He 0.66 0.57 0.07 0.36 - - 0.59 0.4 0.71 0.31
  Ho 0.66 0.62 0.07 0.31 - - 0.6 0.47 0.8 0.13
TOA2 N 16 16 16 16 16 16 16 16 16 16
  Nall 5 3 2 2 2 1 5 2 4 2
  FIS -0.277 +0.540 -0.429 +1 - - +0.080 -0.050 -0.149 -
  He 0.69 0.54 0.44 0.31 0.06 - 0.34 0.42 0.54 0.06
  Ho 0.88 0.25 0.63 - 0.06 - 0.31 0.44 0.63 0.06
TOA3 N 15 15 15 15 15 15 15 15 15 15
  Nall 5 3 1 3 1 1 4 2 3 4
  FIS -0.102 -0.426 - +0.691 - - +0.142 -0.359 -0.011 -0.094
  He 0.73 0.52 - 0.42 - - 0.62 0.5 0.66 0.31
  Ho 0.8 0.73 - 0.13 - - 0.53 0.67 0.67 0.33
FAA N 11 11 11 11 11 11 11 11 11 11
  Nall 5 2 1 2 1 1 6 2 3 1
  FIS -0.165 +0.107 - +0.643 - - +0.241 0 -0.026 -
  He 0.47 0.51 - 0.25 - - 0.71 0.45 0.18 -
  Ho 0.55 0.46 - 0.05 - - 0.55 0.45 0.18 -
MOG N 10 10 10 10 10 10 10 9 9 9
  Nall 4 4 2 2 1 1 5 2 4 2
  FIS -0.000 +0.316 -0.200 +1 - - -0.248 -0.455 -0.347 -
  He 0.6 0.43 0.34 0.19 - - 0.57 0.47 0.68 0.11
  Ho 0.6 0.3 0.4 - - - 0.7 0.67 0.89 0.11
MOG2 N 18 18 18 18 18 18 18 18 18 18
  Nall 5 4 2 3 1 2 4 2 5 1
  FIS -0.012 +0.163 -0.259 +0.785 - - +0.092 +0.329 +0.101 -
  He 0.66 0.53 0.36 0.25 - 0.06 0.43 0.24 0.62 -
  Ho 0.67 0.44 0.44 0.06 - 0.06 0.39 0.16 0.56 -
ILP N 7 7 7 7 7 7 7 7 7 7
  Nall 3 2 1 3 1 1 3 2 2 1
  FIS +0.217 +0.478 - +1 - - -0.200 +0.625 +0.368 -
  He 0.54 0.53 - 0.61 - - 0.49 0.36 0.44 -
  Ho 0.43 0.29 - - - - 0.57 0.14 0.29 -
TA N 4 4 4 4 4 4 4 4 4 4
  Nall 5 3 1 2 1 2 2 3 3 1
  FIS +0.143 -0.091 - - - - +0.143 -0.600 -0.286 -
  He 0.85 0.48 - 0.24 - 0.25 0.58 0.68 0.6 -
  Ho 0.75 0.5 - 0.13 - 0.25 0.5 1 0.75 -
NF N 19 19 19 19 19 19 19 19 19 19
  Nall 4 6 1 2 1 2 2 2 4 1
  FIS -0.093 -0.024 - +0.780 - - -0.059 -0.029 -0.026 -
  He 0.63 0.62 - 0.24 - 0.05 0.15 0.1 0.56 -
  Ho 0.68 0.63 - 0.05 - 0.05 0.16 0.1 0.57 -
FF N 13 13 13 12 13 13 13 13 13 13
  Nall 5 3 1 2 1 2 4 2 5 1
  FIS +0.050 +0.177 - +1 - - -0.043 -0.263 +0.072 -
  He 0.65 0.55 - 0.39 - 0.08 0.22 0.37 0.66 -
  Ho 0.62 0.46 - - - 0.08 0.23 0.46 0.61 -
Total N 279 279 279 278 279 279 265 250 250 250
  Nall 10 8 5 6 3 3 9 5 12 7
  FIS -0.082 0.041 -0.183 0.675 0.663 0.01 0.054 -0.102 0.02 0.102
  He Avg 0.66 0.50 0.29 0.37 0.11 0.12 0.49 0.42 0.58 0.18
  Ho Avg 0.70 0.47 0.32 0.17 0.06 0.10 0.48 0.48 0.58 0.18
  1. Sample size, microsatellite variability, in breeding coefficient (FIS), Observed and expected heterozygosity for A. polynesiensis samples collected in the Society Islands, French Polynesia, 2005-2008. N = sample size, Nall = number of alleles, FIS = the inbreeding coefficient, which is a measure of the reduction of heterozygosity of a subpopulation due to non-random mating, * = significant (P < 0.026) after a Bonferroni correction for heterozygote deficit using a Fisher's exact test
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