Methylation sensitive restriction assay. Genomic DNA of B. glabrata: Brazilian strain (B. glabrata Bre) and Guadeloupian strain (B. glabrata Gua), of positive controls: O. mykiss and HeLa cells and of negative controls: S. mansoni (miracidia) was digested with the Msp I enzyme, its methylation sensitive isoschizomer Hpa II or was not digested (ND). 200 ng of DNA were loaded per well. Digestion profile was observed on an ethidium bromide stained 1% agarose gel. Lad: 1 kb DNA ladder (promega), lowest band 1500 bp, highest band 10000 bp.