Figure 1From: 5-methyl-cytosine and 5-hydroxy-methyl-cytosine in the genome of Biomphalaria glabrata, a snail intermediate host of Schistosoma mansoniMethylation sensitive restriction assay. Genomic DNA of B. glabrata: Brazilian strain (B. glabrata Bre) and Guadeloupian strain (B. glabrata Gua), of positive controls: O. mykiss and HeLa cells and of negative controls: S. mansoni (miracidia) was digested with the Msp I enzyme, its methylation sensitive isoschizomer Hpa II or was not digested (ND). 200Â ng of DNA were loaded per well. Digestion profile was observed on an ethidium bromide stained 1% agarose gel. Lad: 1Â kb DNA ladder (promega), lowest band 1500Â bp, highest band 10000Â bp.Back to article page