Alignment from 3′ to 5′ end of the COI target region for probes. The alignment of An. funestus for the comparison were originated from GenBank (access No. AY423059). Mal and Moz represent Malawi and Mozambique respectively. Dots represent identity with respect to the An. funestus sequences. AG, CT and AT indicate polymorphic positions. The square indicates the sequences of the probes for clades I and II.