GMD and GMER protein expression in larval and adult Schistosoma mansoni . Membrane-purified chicken IgY antibodies (see in Additional file 4: Figure S3) were used in western blot analyses of 2-day in vitro-cultivated primary sporocyst subcellular protein extracts (A). Four enriched fractions were examined: (F1) cytosol, (F2) membrane/membrane organelle, (F3) nucleus, and (F4) cytoskeleton. Blots indicate the presence of GMD and GMER only in the cytosolic fraction. Next, cytosolic fractions were used to compare GMD and GMER protein expression among mixed-sex adults, miracidia and 2- and 10-day in vitro-cultivated primary sporocysts (B). In both experiments, total protein was visualized in-gel by Coomassie staining.