Figure 2

Ethidium bromide-stained agarose gel showing multiplex PCR assay for molecular identification. M: Maker; Positive control products from Cx. pipiens complex are shown in lanes1-7. Lane 1 (Cx. pipiens pipiens from Urumchi, Xinjiang, 610 bp); Lane 2 (Cx. pipiens quinquefasciatus from Dali, Yunnan, 274 bp); Lane 3 (Cx. pipiens pallens from Beijing, 478 bp); Lane 4 (Cx. pipiens pipiens &Cx. pipiens pallens, 1 ul ofeach DNA, 610 bp &478 bp); Lane 5 (Cx. pipiens pipiens &Cx. pipiens quinquefasciatus, 1 ul of each DNA, 610 bp&274 bp); Lane 6 (Cx. pipiens quinquefasciatus&Cx. pipiens pallens, 1 ul of each DNA,274 bp&610 bp); Lane 7 (Cx. pipiens pipiens&Cx. pipiens pallens&Cx. pipiens quinquefasciatus, 0.67 ul of each DNA, 610 bp&274 bp&478 bp), respectively. Mosquitoes from Lhasa city and other provinces are shown in lanes 8–22, respectively. Lane 23, negative control. Outside lanes are 100 bp DNA ladders. bp = basepaires.