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Table 2 KWAG-base/KWAG-perm primer information for qPCR (F = forward, R = reverse)

From: DDT and pyrethroid resistance in Anopheles arabiensis from South Africa

Gene

Primer sequence

Primer concentration

Annealing temperature

CYP6AG2

F 5′- TTG TGC TGC CGT ACT ATT CG-3′

2.0 μM

59.4°C

 

R 5′- TAC TAT CGC CCG TCT CAC CT -3′

  

TPX2

F 5′- GGA TGT TTG TGG GGA ATA CG -3′

3.5 μM

56.3°C

 

R 5′- TGT GCG ATT AGC CTC CTC TT-3′

  

18S

F 5′- TAC CTG GGC GTT CTA CTC -3′

a

b

 

R 5′- CTT TGA GCA CTC TAA TTT GTT C -3′

  
  1. aPrimer concentration was the same as that of CYP6AG2 and TPX4 when used as a reference gene in each case.
  2. bAnnealing temperature was the same as that of CYP6AG2 and TPX4 when used as a reference gene for each.