Strategy to amplify segment 6 of domain II of the VGSC gene in Anopheles albimanus . (A) Diagrammatic representation of the design of degenerate and specific primers for An. albimanus [GenBank: KF137581] based on An. gambiae [GenBank: Y13592] and An. punctipennis [GenBank: AY283041]. The identical positions are indicated by an asterisk and mutation site is enclosed by a box. Intron position is indicated by a black line below the sequence. AAKDRF (5′-AGATGGAAYTTYACNGAYTTC-′3); AAKDRF2 (5′-CATTCATTTATGATTGTGTTTCGTG-′3); AAKDRR (5′-GCAANGCTAAGAANAGRTTNAG-′3). (B) PCR products using degenerate and specific primers. The PCR products were separated on a 2% agarose gel containing ethidium bromide. Lane 1: 50 bp DNA ladder (Novagen); Lane 2: degenerate PCR products (using AAKDRF and AAKDRR primers); Lane 3: negative control of degenerate PCR (H2O); Lane 4: specific PCR product (using AAKDRF2 and AAKDRR primers); Lane 5: negative control of specific PCR (H2O).